Introduction to histopathology
Introduction to histopathology

Start this free course now. Just create an account and sign in. Enrol and complete the course for a free statement of participation or digital badge if available.

Free course

Introduction to histopathology

4.2 Magnification and scale bars

When you see micrographs in older text-books, a magnification is usually stated in the legend (e.g. x100). Strictly, this should mean that the magnification of the illustration in the book is 100-fold larger than the original item. However, there is occasionally some ambiguity. For example, the statement can mean that the picture was taken using a microscope set with a 100x magnification (10x objective, 10x eyepiece). Since the light path to the camera is not the same as the light path to the eye (which passes through the eyepieces), these magnifications are not meaningful. Moreover publishers may increase or decrease the size of a micrograph to fit the available space. The stated magnification in the legend should then be corrected, but often it is not.

For these reasons, the use of scale-bars has replaced a statement of magnification. A scale bar, corresponding to a convenient unit of length, is added to the image taken by the camera, and is then an integral part of that image. If the image is increased or reduced in size thereafter, the scale bar changes in proportion, so that it is always possible to see the correct size of the cells or tissue.

S210_3

Take your learning further371

Making the decision to study can be a big step, which is why you'll want a trusted University. The Open University has 50 years’ experience delivering flexible learning and 170,000 students are studying with us right now. Take a look at all Open University courses372.

If you are new to university level study, we offer two introductory routes to our qualifications. Find out Where to take your learning next?373 You could either choose to start with an Access courses374or an open box module, which allows you to count your previous learning towards an Open University qualification.

Not ready for University study then browse over 1000 free courses on OpenLearn375 and sign up to our newsletter376 to hear about new free courses as they are released.

Every year, thousands of students decide to study with The Open University. With over 120 qualifications, we’ve got the right course for you.

Request an Open University prospectus371