Long description

The figure consists of three parts, Figures 15a to c, that illustrate a confocal microscope, optical section processing, and a micrograph showing a 3D reconstruction of a blood vessel within the brain tissue.

Figure 15a: a photo shows a confocal microscope. The microscope is connected to a computer system on the right. The desktop displays a fluorescent micrograph, captured by the confocal microscope, of a sample positioned on a slide beneath the microscope.

Figure 15b: an illustration shows the optical sectioning process comprising three parallel transverse sectioning of a blood vessel (green) along the horizontal plane.

Figure 15c: a fluorescence micrograph shows a cylindrical blood vessel emitting green fluorescence and surrounded by astrocytes emitting blue and red fluorescence. The scale bar reads 10 micrometres.