Exploring cells with digital fluorescence microscopy
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Contents

  • Introduction
  • Learning outcomes
  • 1 Introduction to microscopy
    • 1.1 Why do we need microscopy?
    • 1.2 Magnification and resolution are important when studying details
  • 2 Types of microscopy
    • 2.1 Components of a light microscope
    • 2.2 Fluorescence (light) microscopy
    • 2.3 Components of a fluorescence microscope
    • 2.4 Electron microscopy
  • 3 The science behind fluorescence and its applications in microscopy
    • 3.1 Fluorescence is activated by light
    • 3.2 The colour of the emitted light depends on the fluorescent molecule
  • 4 Applying fluorescence microscopy to make cells colourful
    • 4.1 Journey into a cell
    • 4.2 Using dyes (or probes) to stain structures
    • 4.3 Immunolabelling
    • 4.4 Expression of fluorescent proteins
    • 4.5 Similarities and differences between the three types of microscopy
  • 5 Why is fluorescence microscopy so versatile?
    • 5.1 Fluorescence microscopy allows observing specific structures individually and combined
    • 5.2 Fluorescence microscopy allows visualising dynamic processes
    • 5.3 Specialised applications: Confocal microscopy
    • 5.4 Specialised applications: Studying molecular dynamics
    • 5.5 Specialised applications: Super-resolution microscopy
  • 6 Inspecting structures in a 3D cell
  • 7 Using the digital fluorescence microscope
  • 8 Quiz
  • Conclusion
  • Further reading
  • Acknowledgements
  • Glossary

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