# 8 Quiz

Now that you’ve reached the end of this course, here are some quiz questions to test and consolidate your learning.

## Question 1

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## Question 2

Choose which information is better to judge the size of a cellular structure in a micrograph.

(Note: Information for answering this question was provided in Section 2.)

Scale bar Correct. A scale bar allows to directly judge the size of structures in an image, independent of any magnification that might have happened after the image was take (e.g. resolution of the screen, size of a printed image).
Value of magnification Incorrect. The magnification depends on the objective with which the image was taken, but also on other factors that might be applied after the micrograph was taken. For example, the size of a printed image or the resolution of a computer screen affect the magnification of structures in a micrograph.

## Question 3

True or false?

Light is the only medium used in microscopy to obtain an image from a sample.

(Note: Information for answering this question was provided in Section 2.4.)

True
False Light is commonly used, but electron microscopy uses electron beams to visualise the sample.

## Question 4

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## Question 5

True or false?

The wavelength by which a fluorophore emits fluorescence is shorter than the wavelength used for excitation.

(Note: Information for answering this question was provided in Section 3.)

True
False

## Question 6

double check first sentence here – both answers were listed as dyes but I think the second should’ve been indicators? (I’ve adjusted accordingly)
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OpenLearn - Exploring cells with digital fluorescence microscopy
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