Acknowledgements

This free course was written by Dr Corrado Minetti.

Except for third party materials and otherwise stated (see terms and conditions), this content is made available under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 Licence.

The material acknowledged below is Proprietary and used under licence (not subject to Creative Commons Licence). Grateful acknowledgement is made to the following sources for permission to reproduce material in this free course:

Text

Activity 2: Extract from: Kilonzo-Nthenge, A., Rafiqullah, I., Netherland, M. Jr., Nzomo, M., Mafiz, A., Nahashon, S. and Hasan, N. A. (2024) ‘Comparative metagenomics of microbial communities and resistome in southern farming systems: implications for antimicrobial stewardship and public health’, Frontiers in Microbiology, 15, p. 1443292. doi: 10.3389/fmicb.2024.1443292. an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0)

Activity 3: Extract quotes from: open access article Nousias, O., McCauley, M., Stammnitz, M. R., Farrell, J. A., Koda, S. A., Summers, V., Eastman, C. B., Duffy, F. G., Duffy, I. J., Whilde, J. and Duffy, D. J. (2025) ‘Shotgun sequencing of airborne eDNA achieves rapid assessment of whole biomes, population genetics and genomic variation’, Nature Ecology & Evolution, 9(6), pp. 1043–1060. doi: 10.1038/s41559-025-02711-w.

Figures

Course image: Pixabay

Figure 1: (interactive) Pixabay

Figure 2: adapted courtesy: AAT Bioquest Home | AAT Bioquest

Figure 3: The History of DNA Sequencing in Microbe Notes Metagenomics: Principle, Types, Steps, Uses, Examples, Diagram July 6, 2024 by Sanju Tamang https://microbenotes.com/metagenomics/

Figure 4: in Pavlopoulos, G.A., Oulas, A., Iacucci, E. et al. Unraveling genomic variation from next generation sequencing data. BioData Mining 6, 13 (2013). https://doi.org/10.1186/1756-0381-6-13

Figure 5: compiled using public domain illustration and Clipart

Figure 6: (interactive) Pixabay

Figure 7: Schematic of how AMR bacteria can transmit between humans, animals, and the environment Figure 1. in Antimicrobial Resistance in New Zealand—A One Health Perspective by Isabelle Pattis, Louise Weaver, Sara Burgess, James E. Ussher, Kristin Dyet https://www.mdpi.com/2079-6382/11/6/778

Figure 8: (interactive) left to right:

• U.S. Air Force photo by Jill Pickett https://commons.wikimedia.org/wiki/File:221205-F-KN521-0064.jpg
• U.S. Navy photo https://commons.wikimedia.org/wiki/File:US_Navy_050910-N-2653P-132_Forward_Deployable_Preventive_Medicine_Unit_(FDPMU)_East,_removes_a_Light_Trap_provided_by_the_Centers_for_Disease_Control_(CDC)_from_a_tent_city_area_on_board_NAS_JRB_New_Orleans.jpg
• Mongan, A. E., Tuda, J. S. B. & Runtuwene, L. R. (2020) ‘Portable sequencer in the fight against infectious disease’, Journal of Human Genetics 65, pp. 35–40 . https://doi.org/10.1038/s10038-019-0675-4 https://creativecommons.org/licenses/by/4.0/deed.en

Figure 9: (interactive) left to right:

• Wouter Hagens https://commons.wikimedia.org/wiki/File:Eichhornia_crassipes_C.jpg
• Bj.schoenmakers https://commons.wikimedia.org/wiki/File:Dreissena_polymorpha_(Zebra_mussel),_Arnhem,_the_Netherlands.jpg
• Gordon H. Rodda https://commons.wikimedia.org/wiki/File:Brown_tree_snake-Boiga_irregularis.jpg

Figure 10: In Amanda Mikko1 · Jose Antonio Villegas2 · Daniel Svensson2 · … · Mats Forsman3 · Anna Berlin5 · Per Stenberg2,3,6 https://www.cell.com/iscience/fulltext/S2589-0042(25)01173-3?uuid=uuid:ab4be347-7063-487c-9438-131757c7e079 Volume 28, Issue 7112912 July 18, 2025 Copyright: © 2025 The Author(s). Published by Elsevier Inc. Deed - Attribution 4.0 International - Creative Commons

Every effort has been made to contact copyright owners. If any have been inadvertently overlooked, the publishers will be pleased to make the necessary arrangements at the first opportunity.

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