Gradient diffusion test

A simple means of determining MIC. It uses thin plastic test strips that are impregnated on the underside with a dried antibiotic concentration gradient and are marked on the upper surface with a concentration scale. These are placed on an inoculated agar plate. After overnight incubation, the tests are read by viewing the strips from the top of the plate. The MIC can be read at the point where the zone of inhibition meets the test strip.

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