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D

Double disk synergy testing (DDT)

A test most frequently used to detect ESBL. It involves applying a disk containing the test antibiotic (mostly a cephalosporin) next to a disk containing an inhibitor (mostly clavulanic acid) on an inoculated agar plate. If ESBL is present, there is a distorted zone on the side of the test antibiotic nearest the inhibitor disk. Standard AST disks are commonly used for this.