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G

Genotypic methods

Methods based on the detection of genes.


Gradient diffusion test

A simple means of determining MIC. It uses thin plastic test strips that are impregnated on the underside with a dried antibiotic concentration gradient and are marked on the upper surface with a concentration scale. These are placed on an inoculated agar plate. After overnight incubation, the tests are read by viewing the strips from the top of the plate. The MIC can be read at the point where the zone of inhibition meets the test strip.


Gram-negative

Bacteria that do not retain crystal violet dye in the Gram-staining test.


Gram-positive

Bacteria with a thick peptidoglycan layer in their cell wall that is stained blue–purple by the Gram stain dye.



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