8 Summary

In this module you have learned about AST and other phenotypic methods of testing for antibiotic resistance. You have seen the importance of quality procedures to ensure that the results of these tests are uniform, consistent, repeatable and reliable. You have also been introduced to the EUCAST and CLSI internationally recommended standards that allow data from different laboratories to be compared.

You should now be able to:

• describe the principles of AST
• understand the concepts of phenotypic methods for AST
• understand the process of disk diffusion testing and the relevant quality measures needed
• understand the principles of other phenotypic methods, including automated methods
• understand that the method used may depend on the organism being tested
• understand the concept of MICs and breakpoints
• recognise relevant international guidelines, and know where to find them and when to use them
• recognise that additional testing may be needed to identify/confirm resistance mechanisms
• recognise the importance of procedures designed to ensure the quality of AST testing in your workplace.

Now that you have completed this module, consider the following questions:

• What is the single most important lesson that you have taken away from this module?
• How relevant is it to your work?
• Can you suggest ways in which this new knowledge can benefit your practice?

When you have reflected on these, go to your reflective blog [Tip: hold Ctrl and click a link to open it in a new tab. (Hide tip)]   and note down your thoughts.