4.2.3 Activity 6: Culture media in your workplace

Activity 6: Culture media in your workplace

Timing: Allow about 10 minutes

Complete Table 4 based on the types of media you use in your laboratory. What considerations guide your laboratory’s choice of media?

Table 4 Media used in your workplace
Name of medium Use Category
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Discussion

You may use other media not shown in the table.

The media available to your laboratory will depend on factors such as local suppliers, transport and logistics, preferences in your country/region, and cost.

There may be more up-to-date media than the ones you are using. Ready-made media save time in pouring, sterilising and quality control (QC).

Table 4 Media used in your workplace
Name of medium Use Category
MacConkey To select for Gram-negatives and differentiate lactose fermenters from non-lactose fermenters Solid selective differential
Blood agar Enriched with 5% whole blood. For fastidious organisms. Is also differential by the haemolysis patterns for some organisms Solid enriched differential
Tetrathionate broth Primary selective broth used for Salmonella Broth, selective

4.2.2 Transport media

4.3 Incubation of bacterial cultures