This free course provides an introduction to microscopy and the operation of a simple light microscope, of the type found in histology courses and teaching laboratories. It outlines different methods used for preparing and staining tissue sections for microscopy, and explains how different stains can be used to identify particular cells, pathogens and anatomical structures.
Course learning outcomes
After studying this course, you should be able to:
identify and understand the principle components of a light microscope
understand in outline, the principles of optics that apply to light microscopes
set up and use a light microscope
understand why different staining procedures are used on tissue sections
understand the principles of immunohistochemistry.