2.5 Using IHC to identify B cells and T cells
T cells and B cells are the two major subtypes of lymphocyte. B cells may ultimately differentiate into antibody-producing cells, while T cells perform many functions in immune defence.
Within lymph nodes B cells tend to distribute to the follicles, and T cells to areas of the paracortex. The distribution can be readily seen by IHC staining of the cells with appropriate antibodies against their cell surface markers: CD3 for T cells or CD20 for B cells.
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Look at Slides 13 and 14 from the ‘Week 2’ category.
Spend a few moments looking at where the T cells and B cells are distributed. Then compare these lymph node sections with the H&E stained section in Slide 12.