Lesson Overview
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This session will review how to make a recombinant genomic DNA library and how to use this library to find a specific gene. This session will outline using a library to clone a gene by complementation of a mutant phenotype.
Learning Objectives
- To understand what a recombinant genomic DNA library is and how it is constructed.
- To identify how to use a genomic library to identify a gene of interest.
- To understand how to clone the gene of interest by complementation of a mutant phenotype.
- To understand the limitations of a genomic library.