This session will outline methods to analyze genes identified through recombinant DNA technologies.

Learning Objectives

• To understand what an agarose gel is and how to use agarose gel electrophoresis to analyze DNA molecules.
• To understand the basic mechanism of DNA sequencing by the dideoxy chain termination method.
• To know that there is a vast database containing the DNA sequence of the entire genomes for many different organism, and understand why this is useful.
• To understand the basic mechanism of the polymerase chain reaction (PCR) method of amplifying DNA and know when to use this technique.